《植物生理学报》 2009, 45(10): 972-976

油菜愈伤组织中烯丙基硫苷的积累和某些外源碳氮化合物对其含量的影响

吴媛媛^1,2, 马绍英^1,2, 李胜^1,2,*, 张真^1,2, 刘媛^1,2, 张青松^1,2

1 甘肃农业大学生命科学技术学院植物细胞工程研究室, 兰州730070; 2 兰州汇通生物科技有限公司, 兰州730000

通信作者：李胜；E-mail: lish@gsau.edu.cn；Tel: 0931-7631547

摘　要：

油菜愈伤组织在培养中生长34 d的增殖量最大, 增殖倍数为7.08倍, 烯丙基硫苷的含量在第6天最高; 外源L-缬氨酸明显促进烯丙基硫苷的积累, 其浓度为2和4 mmol·L-1时烯丙基硫苷含量分别达到98.5和97.40 mg·g-1 (DW); 蔗糖最有利于油菜愈伤组织的生长和其中烯丙基硫苷的积累, 浓度以30 g·L-1的蔗糖为最佳。

关键词：油菜; 烯丙基硫苷; 前体物质; 糖源; 愈伤组织

收稿：2009-08-19   修定：2009-09-27

Accumulation of Sinigrin in Brassica campestris L. Callus and Effects of Some Exogenous Carbon and Nitrogen Containing Compounds on Its Contents

WU Yuan-Yuan^1,2, MA Shao-Ying^1,2, LI Sheng^1,2,*, ZHANG Zhen^1,2, LIU Yuan^1,2, ZHANG Qing-Song^1,2

¹Laboratory for Plant Tissue Culture, College of Life Science & Technology, Gansu Agricultural University, Lanzhou 730070, China; ²Lanzhou Huitong Biological Science & Technology Ltd., Lanzhou 730000, China

Corresponding author: LI Sheng; E-mail: lish@gsau.edu.cn; Tel: 0931-7631547

Abstract:

It was studied by callus culture to accumulation dynamics of sinigrin in rape (Brassica campestris) callus and effects of some exogenous carbon and nitrogen containing compounds on sinigrin contents. The extreme proliferation multiples of callus was 7.08 in the 34th day of culture. The sinigrin content reached to 109.04 mg·g^-1 (DW) in the highest at the 6th day. When the addition concentrations of L-valine were 2 mmol·L^-1and 4 mmol·L^-1, the sinigrin contents of rape callus were 98.5 and 97.40 mg·g^-1 (DW), respectively, which were promoted obviously. Sucrose was the optimal carbon source for rape callus multiplication and sinigrin accumulation with the best addition concentration of 30 g·L^-1.

Key words: Brassica campestris; sinigrin; precursors; carbon source; callus